Author

Yuliana Retnowati

Subject

- Sains

Abstract

The aim of the study was to obtain and determine the mercury utilizing bacteria form the contaminated river sediment by gold mining wastes. Mercury utilizing bacteria were isolated through batchwise enrichment culture techniques using modified nutrient broth containing 0.1 mgL-1 methyl mercury chloride (CH3HgCl). Selection of isolates was carried out through the growth experiment based on their abilities to grow on different methyl mercury concentration, in terms of generation time (g) and the specific growth rates (µ). Three types of bacteria showing the highest, moderate, the lowest generation time were selected and purified for further identify experiments. The selected isolates were identified using standard methods. The result revealed that fourteen mercury utilizing bacteria were successfully isolated from river sediment (133.5x106 CFU/ml). Only four isolates were able to grow on growth medium containing 2-4 mg L-1 at generation times 1.3-5.5 h and specific growth rates of 0.1-0.5 generation h-1. The four selected isolates were identified as Baccillus sp. (strain YLN002), Alcaligenes sp. (strain YLN004 and strain YLN005) and pseudo-monas sp. (strain YLN006). Keyword: Isolation, Identification, mercury utilizing bacteria

Publisher

Lembaga Penelitian Universitas Negeri Gorontalo

Contributor

-

Publish

2011

Material Type

ARTIKEL

Right

-