Sunarto Kadir
- Kesehatan
Most of probiotics are used as a therapeutic modality and food supplement for improving the system of body immunity. However, it has not been known that the mechanism of probiotic immunomodulation effect at the system of elderly people’s immunity. Moreover, this study aimed at analyzing the effect of probiotic Lactobacillus plantarum IS-10506 toward the immune response of CD4+ and IgA in infection model in giving LPS to the mice (Rattus norvegikus) strain elderly Wistar instead of healthy elderly mice. Furthermore, this study is an experimental study by using the randomized post test only control group design. The subject of this study was male mice that consisted of 4 groups based on treatment given which were a group that was given placebo (control) in first day until ninth day, a group that was given LPS in first day, a group that was given probiotic in third day until ninth day, and a group that was given LPS in first day and probiotic in third day until ninth day. All of groups were euthanized in tenth day. An examination of immunohistochemistry at the small intestine used specific monoclonal antibody which was mouse anti-Rat CD4+ and IgA. A new finding based on this study was giving probiotic and LPS could increase the content of IgA at the elderly mice, and it could be proven scientifically that IgA as the adaptive immunity in secondary phase, with second stimulation in giving probiotic was occured switching IgM and IgG became IgA that had high protective power toward pathogen. In conclusion, giving probiotic Lactobacillus plantarum IS-10506 and LPS could affect to the rise of immune response that appeared from the expression of immune response such as the rise of CD4+ and IgA in homeostasis condition.
Research Journal of Pharmaceutical, Biological and Chemical Sciences (RJPBCS) 2015
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