Author

Agus Bahar Rachman

Subject

- Peternakan

Abstract

41 samples of DNA genome of kacang goat blood in the Kota Gorontalo (21) and Bone Bolango Regency (20) has been extracted in the Faculty of Animal Science, Biotechnology Laboratory of Integrated Hasanuddin University. Amplification and Genotyping is applied by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the restriction enzyme HaeIII. The analysis of IGF-1 gene from both locations shown two kinds of alleles (A = 0.951, B = 0.048) and two kinds of genotype (AA = 0.902, AB = 0.975), observed heterozygosity (Ho) = 0.097 and expected heterozygosity (He) = 0.093. Partially gene IGF-1 in the Kota Gorontalo has two kinds of alleles (A = 0.952 and B = 0.047) and two genotypes (AA = 0.904, AB = 0.095), Ho = 0.095 and He = 0.092. Instead IGF-1 gene in Bone Bolango Regency have two kinds of alleles (A = 0.95, B = 0.05) and two genotypes (AA and AB = 0.90 = 0.10) with Ho = 0.10 and He = 0.097. Based on the results, it can be concluded that IGF-1 gene in kacang goat of Kota Gorontalo and Bone Bolango regency are polymorphic so it can have opportunity for doing selection. Keywords: Genetic Diversity, Insulin-Like Growth Factor-1, Kacang Goat

Publisher

UB (University of Brawijaya) Press

Contributor

Fahrul Ilham, Safriyanto Dako, Agus Bahar Rachman, Muhammad Ihsan Andi Dagong, Lellah Rahim

Publish

2016

Material Type

PROSIDING

Right

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