SCIENTIFIC WORK

Author
Femy Mahmud Sahami
Subject
- Perikanan
Abstract
Abstract. Nike is Gobioidei fish identified at the post-larval and juvenile stages whose habitat is still in the water of sea before they migrate to the fresh water, grow up, and spawn to fulfill their life cycle as an amphidromus species. This fish is very popular, favored by people, and has become an important economic commodity in Gorontalo. One of the biggest Nike fishing locations is Gorontalo B ay. This is very important to explore the types of species composing the Nike fish clusters in their contribution to fish biodiversity. Accordingly, this study aims to identify the species composers of Nike fish in Gorontalo Bay waters based on their morphometric and molecular characters. 2,523 samples were collected from fishermen’s catches during three periods of their appearance in Gorontalo Bay waters to the estuary areas of Bone Bolango River from January to March 2019. The samples were then grouped based on their similarity of melanophore patterns and morphometric characters of 10 units were then measured. This study found 20 different groups of melanophore pattern, 15 of them were new melanophore patterns. DNA samples from each group of new melanophore patterns were then isolated for molecular analysis. The data of morphometric characters were analyzed for its differentiators, while the DNA was analyzed using BLAST (Basic Local Alignment Search Tools) from NCBI. The results of morphometric analysis grouped the 20 melanophore patterns into three separate clusters that were confirmed through molecular analysis. The results of Gen Cytochrome Oxidase I (COI) sequences of mitochondrial DNA indicate that the Nike fish clusters in Gorontalo Bay waters have a high level of diversity with the discovery of six species (i.e. S. parvei, S. cynocephalus, S. longifilis, S. lagocephalus, and Stiphodon semoni) generated Gobiidae family and Belobranchus belobranchus species generated from Eleotridae family. It completes the data of the diversity of Nike fish composers in Gorontalo Bay waters which is very significant as the reference for inventorying and identifying the types of Nike fish in other estuary areas and adult amphidromous Gobies in Gorontalo rivers.
Publisher
B I O D I V E R S I T A S
Contributor
-
Publish
2020
Material Type
ARTIKEL
Right
-
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